Aerial conidia were one of key ingredient for formulation and application of fungal biocontrol agents.Statistical optimization strategy of technology parameters was adopted to maximize the conidia production through series fermentation chamber which have been previously developed to produce high-quality conidia powder.Beauveria bassiana ChB9 isolate was evaluated as fermentation candidate.Three important fermentation factors including culture substrates,sterilization conditions and rates of substrate weight and windrowing area(g∶ cm2) at varied combinations were used to study the optimal parameters required for series fermentation.Conidia powder yield,mycelium powder yield and rice consumption rate under different factors combination were compared.The combination japonica rice substrate with 105℃ 30 minutes sterilization under 1∶ 2 weight/area rate conferred best conidia powder yield of 17.56±2.79g per 1 kg rice substrate.Those results might be not only advantageous for further studies on laboratory-scale conidia production of biocontrol agents based on series chamber system,but also guidable for optimization of technology parameters on other substrate fermentation.