In order to obtain the cultivation conditions of kombucha fermentation for litchi juice,effects of fermentation conditions including kombucha inoculation concentration,initial total suluble solid content,fermentation temperature,and initial pH value of litchi juice on acid content,pH,total suluble solids content,reducing sugar consumption rate of the fermented litchi juice were investigated.Response surface methodology and desirability function approach was applied to obtain the optimal cultivation conditions.Results showed that the kombucha cultivation conditions for litchi juice were selected as 10% of inoculums concentration,14°Brix of initial soluble solid content,30℃ of fermentation temperature,after 6 days of kombucha fermentation of litchi juice,the content of acid was increased to 2.48g /100 mL,pH value was 3.04.