Food and Fermentation Industries >

2013 , Vol. 39 >Issue 07: 18 - 23

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2013.07.027

Food and Fermentation Industries

Screening and identification of thermophilic functional bacteria from high-temperature daqu of sesame flavor liquor

  • YAO Su ,
  • ZHANG Ming-juan ,
  • LIU Yong ,
  • XIN Chun-Hui ,
  • XU Ling ,
  • ZHANG Bo-lin ,
  • CHENG Chi
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Online published: 2013-07-25

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Abstract

The high-temperature daqu of sesame flavor liquor was used as experimental samples.Thermophilic functional bacteria were isolated by culturing at 55℃.Through screening of transparent circle and assaying of the solid-state fermentation enzyme activity,protease production strains were isolated.The strains were identified with 16S rDNA gene phylogenetic analysis.The results revealed that 85 strains of thermophilic bacteria were isolated from samples.The neutral protease activity of M5 was higher than control strain,and the enzyme activity of M5 was 96.06 U / g.The strain M5 was identified as Bacillus subtilis subsp.subtilis through molecular biology methods.The strain M5 can be used as microbial agents to produce high-temperature daqu of sesame flavor liquor.

Key words： sesame flavor liquor;;high-temperature daqu;;neutral protese;;screening;;identification

Cite this article

YAO Su , ZHANG Ming-juan , LIU Yong , XIN Chun-Hui , XU Ling , ZHANG Bo-lin , CHENG Chi . Screening and identification of thermophilic functional bacteria from high-temperature daqu of sesame flavor liquor[J]. Food and Fermentation Industries, 2013 , 39(07) : 18 -23 . DOI: 10.13995/j.cnki.11-1802/ts.2013.07.027

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