In this paper,wheat germ protein(WGP) as raw material was hydrolyzed by protease and bond to Ca2+.The formation of wheat germ peptide-calcium complex can be the fourth-generation calcium supplements.Alcalase could hydrolyze wheat germ protein more easily.The combined amount of calcium reached a highest value of 18mg / g protein at the degree of hydrolysis of 21.5%.Result of SDS-PAGE showed the quick degradation of the high molecular weight subunit in WGP,and showed that the hydrolysis of 50kDa and 32 ~ 34 kDa subunit was intimately related to calcium binding capacity.The hydrolysates with high calcium binding ability mainly contained Glu,Arg,Asp,Gly and 44% of hydrophobic amino acids,in which the molecular weight distribution was lower than 2 000Da.Results of full wavelength scanning and infared spectra revealed that the amino and carboxyl groups were involved in the binding reaction and generated new material-wheat germ peptide-calcium complex.