Using crude propolis from Xishuangbanna of Yunnan as raw materials,different propolis extracts were acquired in 75%,85%,95% ethanol at room temperature and by thermal process.The flavonoid contents of propolis extracts were determined.And the inhibition effect of different propolis ethanol extracts on Pleurotuseryngii and contaminative microorganisms was discussed for the first time.The results showed that the highest flavonoids content was 10.51 mg / mL under the conditions of 75% ethanol extraction by thermal process.Propolis extract had inhibition effect on both Pleurotuseryngii and contaminative microorganisms,the inhibition degree from high to low was as followes: bacteria > Pleurotuseryngii > Mould.Correlation analysis indicated that all of the correlation coefficients on the inhibitory rate of Pleurotuseryngii,Mould and bacteria with the flavonoids content were above 0.8,which showed a good correlation.Addition of the right amount propolis extract can effectively inhibit bacterial pollution in Pleurotuseryngii species preservation and fruit body preservation.