The 4-coumarate: coenzyme A ligase gene(4CL) from Arabidopsis thaliana and resveratrol synthase gene(RS) from Vitis vinifera were cloned into the 2μ-based multicopy drug resistance marker plasmids pRS42K(G418 resistance) and pRS42H(hygromycin resistance).The recombinant plasmids were transformed into EC1118 cells using the LiAc / SS carrier DNA / PEG method.A shaking flask fermentation(25 ℃,150 r / min) of the engineered strain in YPD medium supplemented with 0.1 mmol / L p-coumarate every 24 h was performed.Under these conditions,this strain produced 0.78 mg / L resveratrol within 96 h.The result showed that the A.thaliana 4CL gene and V.vinifera RS gene were expressed in this recombinant strain to produce resveratrol from p-coumarate successfully.