The core technology is the use of chromatophore on the F0F1-ATPase molecular motor biosensor for rapid detection of Shigellasonnei in the food.Specific IpaHprobe were connected with F0F1-ATPase's ε subunit using avidin-biotin system,and then biosensors were constructed,the test samples and negative sample,respectively,were combined with biosensors,to compare their catalytic ATP synthesis after 10 min.Shigellasonnei DNA in the samples could be tested.The amount of ATP synthesis could be detected by measuring the amount of H+,while the amount of H+could be indicated by fluorescence intensity through luciferase-luciferin assay.The results of our experiments showed that optimum conditions for detection were the concentration of chroIpaH was 0.031 mg / mL and the concentration of Shigellasonnei DNA was 40 ng / mL.Results from the detection of actual samples were in good agreement with those from traditional detection methods and PCR detection method.
Zhang Jie
,
Wang Yan-fei
,
Wang Xuan
,
Wang Xiao-jin
,
Gu De-zhou
,
Zhang Hui-yuan
,
Shang Shi-jin
,
Wang Juan
,
Wang Qi
,
Wang Pei-rong
,
Chen Guang-quan
,
Yue Jia-chang
. The study of a detecting technology for Shigellabased on F_0F_1-ATPase molecular motor biosensor[J]. Food and Fermentation Industries, 2013
, 39(08)
: 203
-206
.
DOI: 10.13995/j.cnki.11-1802/ts.2013.08.005
