The effects of nitrogen and different light intensity(35 μmol photons /(m2·s),135μmol photons/(m2·s),280μmol photons/(m2·s)) on the growth,total fatty acid(TFA),arachidonic acid(AA) and pigment composition of Parietochloris incisa was investigated.The results suggested that the cultures showed relatively slow increase in biomass under nitrogen-sufficient and low(135μmol photons /(m2·s)) light intensity.The nitrogen-sufficient and high light intensity [280 μmol photons /(m2·s) ]culture reached the highest biomass at the average rate of 7.23 g / L.An increase in TFA was in company with the accumulation of AA in nitrogen-sufficient medium and certain light intensity.The highest percentage of TFA and AA was 38.02% and 16.10% in the light intensity of 280 μmol photons /(m2·s) or 135μmol photons/(m2·s),respectively.Under nitrogen-sufficient culture,carotenoids content was considerably lower relative to that observed during cultivation on complete medium and did not exceed 9.60 mg / L in all these cases.The highest chlorophyll content reached 150.00 mg / L in the nitrogen-sufficient medium and moderate irradiance cultures.