Two protease-producing marine bacterial strains,named SWJS33 and SWJS22,were isolated from samples of deep sea mud from the South China Sea.The protease in the fermented liquid of these isolate strains was researched.The results showed that proteases which produce by the strains of SWJS33 and SWJS22 could be used in wide ranges of pH and temperature.And they not only have good temperature stability,but also have good salt resistances.Based on the physiological and biochemical characteristics and analysis of 16S rDNA sequence of SWJS33 and WSJS22,the two strains were characterized and identified as species of Bacillus licheniformis and Bacillus amyloliquefaciens.And a new way is expected to the development and utilization of deep-sea microbial protease.