Xylanases from different sources have significant differences in their compositions and properties.In order to develop the industrial applications of xylanase from strain EIM-30,we studied its purification and enzymatic properties.After the crude xylanase from Trichoderma reesei EIM-30 was purified using lyophilization,fractional ammonium sulfate precipitation,Sephacryl S-200 High Resolution gel chromatography and HiTrapTMSP(HP) ion chromatography,two purified xylanases were obtained,which were respectively named as Xylanase A and Xylanase B.Xylanase A was purified about 3.58 times with 7.02% recovery of enzyme activity,and Xylanase B was purified about 3.29 times with recovery of 28.29%.SDS-PAGE showed that the molecular weight of Xylanase A and Xylanase B was 29.6 ku and 20.9 ku,respectively.The enzymatic properties showed that the optimum temperature of Xylanase A and Xylanase B was 55 ℃ and 60 ℃,respectively.The optimum pH of Xylanase A and Xylanase B were both 5.0.When the temperature was below 50 ℃ and the pH was in the range from 3.5 to 7.0,Xylanase A and Xylanase B both exhibited excellent stability.Xylanase A could be enhanced by Mn2 +and Tris,but inhibited by Fe2 +,Cu2 +and SDS.Xylanase B could be enhanced by Mn2 +while inhibited by Zn2 +,Ca2 +,Fe2 +,Cu2 +,Ba2 +,Mg2 +and SDS.