In order to obtain the optimum extraction process parameters of total flavonoids from young barley leaves,the effects of liquid-solid ratio and ethanol volumetric fraction and extraction time on flavonoids extraction yield were studied.Young barley leaves were macerated into ethanol solvent.Central Composite Design was applied to optimize extraction process parameters.The results indicate that the influence on the response value of test factors were as follows: liquid-solid ratio > extracting time > ethanol volume fraction.The optimum extraction process parameters were as follows: Liquid-solid ratio 29 mL / g;ethanol volume fraction 90% and extracting time 100 min.Under these conditions,the value of total flavonoids extraction yield was 946.63 μg / g which was very close to the predicted value of 950.36 μg / g.The conclusion is that response surface methodology could be applied to optimize the extraction process parameters of total flavonoids from young barley leaves and the optimized extraction process parameters could be provided a reference for industrial production of flavonoids compound from young barley leaves.