Colorimetry was used to investigate dynamic changes of total flavonoids and polysaccharides content in Fructus ligustri at different collecting periods. The results showed that from August 20th to September 10th,the total contents of flavonoids in Fructus ligustri gradually increased,and from October 26th to December 23rd it gradually decreased. There was a fluctuation between September 10th and October 26th. The total flavonoids in Fructus ligustri exhibited a decreasing trend in polysaccharide content from August 20th to September 10th. However,from September10th to December 23,an opposite pattern was observed and the highest yield of polysaccharides was obtained on December 23,which was 10 folds than that obtained on September 10th. Overall,the total flavonoids showed negative relationship with polysaccharides content in at different collecting periods.