To establish a rapid,sensitive and specific method of polymerase chain reaction( PCR) for detection of Arcobacter species,a pairs of primers Arco / gyrAF and Arco / gyrAF were designed based on gyrA gene. After the amplification by PCR assay,Arcobacter had a positive reaction with 298 bp amplification fragments,while all the other strains just had a negative reaction. The result of sensitivity tests showed that the detection limit for DNA could reach82. 6 pg. Nine of 16 chicken( 56. 25%) samples were positive for Arcobacter using the PCR method. This PCR technique showed definitive advantages in terms of sensitivity and technical simplicity.