An analytical method was developed for the simultaneous determination of 14 organophosphorus pesticides in bamboo shoots by using matrix solid phase dispersion and Gas chromatography determination( MSPD-GC).Bamboo shoot samples were purified by MSPD and eluted by acetone-acetonitrile( 1∶ 1,V / V) were determined by gas chromatography( flame photometric detector). Matrix-matched calibrations were respectively used to quantify the residue concentrations in bamboo shoot samples. The recoveries were in the range of 77. 54% ~ 114. 16% and the relative standard deviation( RSDs) were 1. 23% ~ 5. 01% at 0. 05mg / kg,0. 50mg / kg and 1. 00mg / kg three spiked levels for all analysis. The limits of detection( LOD) were between 0. 008 mg / kg and 0. 024 mg / kg.