It was the first time that vitamin A palmitate was successfully synthesized through catalyzation with immobilized enzyme in solvent-free system. The results showed that the interesterification could take place between vitamin A acetate and methyl palmitate in solvent-free system,wherein the latter was the acyl donors. The conditions for synthesis were optimized as follows: speed was 250 r / min,temperature was 60 ℃,mass fraction of immobilized enzyme was 5%( g / g substrates),molar ratio of methyl palmitate to vitamin A acetate was 1. 3∶ 1. It would take 3h to reach the reaction equilibrium,and the conversion of vitamin A acetate was 26. 93%. In addition,we found that the reaction liquid could possess high ester concentration( 800g / L) and conversion( 80%) if the reaction was carried out in vacuum for another 12h after equilibrium point,.