The synergistic sterilization effect of naringin and pulsed electric fields( PEF) treatment on Saccharomyces cerevisiae was investigated. Results indicated that naringin and PEF both exhibited sterilization effects. The sterilization index of Saccharomyces cerevisiae significantly increased after the treatment of PEF combined application of naringin. Results of the treatment to Saccharomyces cerevisiae by 0. 7 g / L naringin for 5 h along with PEF( 20kV / cm) for 2400,3600 and 4800 μs indicated that the sterilization ratio reached 64. 71%,79. 83% and 93. 87%,respectively,which were almost 2 times to the treatment effect only by PEF and 2. 5 times to only by naringin at the most serious condition.