It was established that the mathematical model of NIR for quantitative analysis of β-glucan in malt by partial least squares. The correlation coefficient of internal cross-validation and predictive value was 0. 8275. Corresponding standard error of calibration was 0. 06. The coefficient of determination between chemical determination and NIR predict reached 0. 827 with 60 malt samples. It was concluded that the β-glucan content in malt could be predicted accurately by NIR model. It could be used in the production for controlling the malt β-glucan content.