The EPS produced by Pantoea agglomerans KFS was a kind of homogeneity heteropolysaccharide comprised of glucose and mannose with a molecular weight of 756 kDa. Hygroscopicity and moisture retention ability of the EPS was investigated under two kinds of humidity conditions with hyaluronic acid( HA) and glycerol as references. Given the excellent antioxidant activity of the EPS,UV-irradiation test was also conducted. It was showed that the EPS was superior to other sources in hygroscopicity,similar to HA and lower to glycerol in moisture retention; The UV-irradiation protective effects on cells of the EPS was related to the EPS concentration and UV radiation time. When the UV exposure time reached 5 min,the survival rates of the E. coli,C. albicans and S. aureus in experimental groups with 4 mg / mL EPS were 48. 49%,36. 73% and 47. 5%,remarkably higher than the rates of 13. 68%,13. 47% and 12. 25% in the control groups,respectively. Therefore,the EPS used as a natural moisturizer and anti-radiation material should have a widely application prospects.