To obtain vanillin mutants with improved vanillin production,an atmospheric and room temperature plasma( ARTP) jet was used to screen high vanillin-producing Amycolatopsis sp. strains by a 96-well culture plate. The positive mutation rate of the strains was 17. 6% under the condition of lethality rate of 61. 71%. A high yield mutant LQ-6 with good genetic stability was obtained. It could produce 6. 78g / L vanillin while a molar yield of ferulic acid arrived 72. 1%,which was 21. 3% higher than that of the original strain. Moreover,the deacetylase activity was enhanced by 43. 2% in LQ-6,while the vanillin oxidase activity was decreased by 38. 7% compared with the original strain.