The essential amino acid L-lysine is one of the most important amino acids applied as supplement in animal feed. As we know,lysine is mainly produced by fermentation process,and raw material cost is the major factor for lysine production. To increase carbon efficiency,more studies are being done either on optimizing the synthesis pathways using existing industrial carbon sources or exploring new carbon sources. In this review,we provide an overview of the metabolic pathways for utilization of different carbon sources,as well as new approaches of metabolic engineering on Corynebacterium glutamicum.