The major antibacterial component in honey is hydrogen peroxide whose antibacterial effect is closely related to the catalase in honey. Hydrogen peroxide exists in honey widely,so it is significant to establish its determination method to evaluate honey's antibacterial property and its intrinsic quality. In this paper,we modified a method to determine the catalase activity of honey referring to the method of Huidobro et al and the catalase activities of 5kinds of unifloral honey samples were determined. The results showed that the catalase activity varied apparently between different kinds of Chinese unifloral honey. Rape honey from Jingzhou,Hubei Province had the highest catalase activity with the value of( 204. 3 ± 3. 8) × 10- 3/( min·g),and the lowest activity appeared in jujube honey from Shijiazhuang,Hebei Province with the value of( 8. 1 ± 0. 6) × 10- 3/( min·g). The catalase activities of Chinese unifloral honey with different types or different producing areas were diverse.