There are lots of fine features on brown shade,such as friendly environment,corrosion resistance,recyclable usage,etc.. It is commonly used as matrices of seed-adhering in aquaculture. This research is the first time to immobilize cells on brown shade. The effect on cyclodextrin glycosyltransferase of the amount of inoculum,immobilization time,and the number of the brown shade was studied in this article. It was found that the immobilized bacterial cells could get higher levels of enzyme production as 5519. 05U / mL under the condition of inoculation of 3%,immobilization time of 1 day,the number of brown shade of 2 pieces. Compared to existed reports on matrices of loofa sponge,loofa sponge-sodium alginate,brown shade-sodium alginate,brown shade has a characteristic with low cost,simple operation,and good operational stability,etc. In the first seven cycles,the activity of the last cycle could be attained as 83. 5% of the first one,thus making the process potentially suitable for industrial scale-up.