Jerusalem artichoke was chosen as the main raw material for the cultivation of Lactobacillus plantarum CX-15. The extraction method of Jerusalem artichoke and optimal ingredient in extractive juice were evaluated through single factor and orthogonal experiment. The result showed that the perfect culture medium composition was Jerusalem artichoke extracted via hot water extraction method with the ratio of solid to liquid 1∶ 10. The extractive juice as basic medium was added with glucose 10 g / L,phytone peptone 40 g / L,MgSO40. 2 g / L,MnSO40. 3 g / L. The viable counts of Lactobacillus plantarum cx-15 reached( 2. 8 ~ 3. 0) × 109CFU / mL after incubated in the optimum medium for 20hours at 34℃,which was 1 times higher than that in the original medium. Compared with MRS medium,the viable counts of Lactobacillus plantarum CX-15 in the two kinds of mediums were at the same level. Using Jerusalem artichoke dry powder as main raw material for lactic acid bacteria fermentation resulted in decreasing the production cost.