This study aimed to analyze the inhibition of different concentration of potassium sorbate on the growth of poisonous Rhizopus stolonifer Vuill. The activated spores were cultured in modified solid culture medium which containing various concentrations of potassium sorbate,then spore germination number was counted,colony size was measured,and then colony color,the morphological characteristics of mycelium and hyphae were observed. The results showed that potassium sorbate significantly inhibit Rhizopus stolonifer Vuill,and the inhibitory effect was significantly related to the applied concentrations. When the concentration was equal or more than 0. 8 g /100mL,the inhibitory effect reached significant level. When the concentration was between 1. 0 g /100mL and 1. 2 g /100mL,79% ~ 90% spores could not germinate,colony and hypha grew slowly and even more,aged and decayed.