Rape bee pollen was powdered and extracted by 90% ethanol under reflux.The dried 90% ethanol extract was then re-dissolved in water and extracted by petroleum ether,ethyl acetate and n-butyl alcohol.We found that petroleum ether extract and ethyl acetate extract has significant antioxidant activity,and can significantly reduce liver malondialdehyde( MDA) content( P < 0.05) and brain monoamine oxidase( MAO) activity( P < 0.05) of the D-galactose induced aging mice.Three linolenic acid compounds were obtained from the petroleum ether extracts,: α-linolenic acid,α-linolenic acid ester,3'-methoxy-α-linolenic acid esters.Three flavonoids were isolated from ethyl acetate extract: kaempferol-3-O-β-D glucopyranosyl( 1→2)-β-D-glucopyranoside,quercetin-3-O-β-D- glucopyranoside,kaempferol-3-O-β-D- glucopyranoside.The results showed that the antioxidant active ingredients in rape bee pollen are mainly linolenic acid compounds and flavonoids.