To develop a method for determination of eighteen amino acids by high performance liquid chromatography and post-column derivation in chicken.Methods petroleum ether was used to remove fat,hydrochloric acid was used to hydrolysis samples.The exchange chromatographic column( 3.0mm × 250mm) was Lithium Ion,mobile phase was Li175,Li750,RG003 as the gradient elution,TRIONE Ninhydrin was used for post-column derivative reagent,detection wavelength was 570 nm.Eighteen kinds of amino acids in 8 ~ 80 μg/mL range showed good linear relationship,recovery rate was high.The amino acid composition of the chicken was also analyzed.The method was simple,fast,accurate,high selectivity,high sensitivity,and stable.It is suitable for all kinds of other meat products and amino acid test.