Food and Fermentation Industries

Optimization of fermentation conditions and kinetics models of batch fermentation for cellulase produced by Rhizopus stolonifer

  • TANG Bin ,
  • XU Zhong-yuan ,
  • LI Song ,
  • CHEN Tao
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Online published: 2014-01-25

Abstract

In this experiment,the filter paper activity was used as index.The single factor experiment and uniform design optimization of medium and culture conditions were carried out.The batch fermentation was carried out on this basis using the Logistic equation and Luedeking-Piret equation establishes dynamic models.The results indicated that the filter paper activity reached 13.16 IU/mL at 84 h,which was increased 149% compared with that before optimization.The highest Endocellulases activity,Cellobiohydrolases activity and β-Glucosidases activity were 45.81 IU/mL,0.537 IU/mL,and 12.45 IU/mL,respectively.The model fitted well.

Cite this article

TANG Bin , XU Zhong-yuan , LI Song , CHEN Tao . Optimization of fermentation conditions and kinetics models of batch fermentation for cellulase produced by Rhizopus stolonifer[J]. Food and Fermentation Industries, 2014 , 40(01) : 85 -90 . DOI: 10.13995/j.cnki.11-1802/ts.2014.01.013

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