DA201-C macro-porous absorption resins were used to purify the fish scales peptides.Then the activities of the fish scales peptides were analyzed.The different elution fractions with different polarity peptides were obtained by changing the alcohol concentration.The ash content of fractions decreased from 1.20% to 0.68% with increased ethanol concentrations,while the content of hydrophobic amino acids and the average hydrophobicity of each eluted fraction increased from 33.83% to 37.67% and from 4.25 kJ/mol to 4.92 kJ/mol respectively.The antioxidant activity,reducing capacity and angiotensin-I converting enzyme( ACE) inhibitory ability increased with peptides concentrations increase.The DPPH· scavenging effect of fish scale peptides decreased when the alcohol concentration increased,while the O 2-· scavenging effect,reducing capacity and ACE inhibitory ability increased.Compared to the antioxidant activity and reducing capacity,the ACE inhibitory ability of the fish scales peptides was stronger at the same concentration.The activities of the fish scales peptides was related with its polarity.