Food and Fermentation Industries >

2014 , Vol. 40 >Issue 02: 83 - 88

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2014.02.001

Food and Fermentation Industries

Overexpression of a lipase gene from Rhizopus niveus in Pichia pastoris and characterization of the expressed enzyme

  • WANG Jian-rong ,
  • LIU Dan-ni ,
  • LI Peng ,
  • LIU Jin-shan ,
  • ZHOU Pin-fa ,
  • CHEN Li-zhi ,
  • NIE Jin-mei ,
  • ZHONG Kai-xing ,
  • LI Yang-yuan
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Online published: 2014-02-25

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Abstract

A lipase gene from Rhizopus niveus( rnl) was isolated by homologous cloning method and subcloned into the vector of pPICZαA. The resultant recombinant plasmid pPICZαA-rnl was digested by SacI and transformed into the competent cells of Pichia pastoris strain X-33 through electroporation. The results showed that the gene was expressed successfully in the Pichia pastoris X-33. The optimal pH and temperature of the recombinant lipase were 35℃ and 8. 5,respectively. The activity of the recombinant lipase was stimulated by 1mmol / L Mn2 +,Ca2 +,K+and 1% Tween-20,Tween-80,TritonX-100. The recombinant lipase showed high activity toward trilaurin( C12).

Key words： Rhizopus niveus; lipase; Pichia pastoris X33

Cite this article

WANG Jian-rong , LIU Dan-ni , LI Peng , LIU Jin-shan , ZHOU Pin-fa , CHEN Li-zhi , NIE Jin-mei , ZHONG Kai-xing , LI Yang-yuan . Overexpression of a lipase gene from Rhizopus niveus in Pichia pastoris and characterization of the expressed enzyme[J]. Food and Fermentation Industries, 2014 , 40(02) : 83 -88 . DOI: 10.13995/j.cnki.11-1802/ts.2014.02.001

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