A simple and rapid SPE-HPLC method for the determination of Gibberellin,Paclobutrazol and Uniconazole residues in fruits was developed. The sample was extracted with 80% methanol-water solution,then was purified by C 18 column and detected in 220 nm with a DAD detector. Linear ranges of three plant growth regulators were in the range of 0. 5 ~ 10 μg / mL with correlation coefficients more than 0. 999. The minimum detection limit of Gibberellin,Paclobutrazol and Uniconazole were 0. 002 mg / kg,0. 04 mg / kg and 0. 02 mg / kg respectively. The average recoveries at spiked concentration levels of 0. 2 ~ 0. 8 mg / kg ranged from 78% to 91%,with relative standard deviations( RSDs) of 2. 4% ~ 5. 4%.