To illustrate the optimal cultural condition for carbonyl reductase production,a novel optimal method based on SVM-PSO theory was proposed. Firstly,four significant impact factors of enzyme production were picked out with the Plackett-Burman experimental design. Then orthogonal design was carried out in order to investigate the effects of the significant impact factors on carbonyl reductase activity. On the basis of orthogonal experiment,SVM model was used for the fitted regression,and SVM-PSO optimal model was constructed for the global optimization. The results of above global optimization were regared as the theoretically optimal fermentation conditions. Under the optimized conditions,carbonyl reductase activity reached 299. 19 U / L,with a 16. 81% increase compared to that before optimization. The result of this experiment showed that the SVM-PSO model was feasible for condition optimization of enzyme production.