Type II collagen was prepared by enzymatic method,and its physical-chemical properties and immunological activities were studied. The results showed that the cartilages from three different sources all contained abundant type II collagen. Chicken sternal cartilage had 81. 54%,pig swine knees cartilage had 81. 31%,and sheep cartilage had 80. 94% type II collagen. The amino acid component analysis and UV spectrum testing verified the extracted collagen was type II collagen. SDS-PAGE showed the extracts had a higher purity. Analysis by Infrared Spectrum and circular dichroism( CD) spectrogram revealed that the extracted type II collagen remained its completed secondary structures. Lymphocyte proliferative responses indicated that the extracted type II collagen possessed immunological activities. The immunological activity of collagen of chicken sternal cartilage was the highest,which attained 52. 91%.