To study separation and purification of total flavones in burdock leaves by polyamide column,and content was determined by spectrophotometry. Water extraction solvent,cellulase extraction solvent,cellulase and 70% alcohol extraction solvent from burdock leaves flew through polyamide column,then total flavones was absorbed and eluted by 70% alcohol,its solution eluting curve,purification and content were determined. The amount of total flavones was the best with cellulase and 70% alcohol. Next one was by cellulase only,the third was by water. Almost all of total flavonoid was eluted by 70% alcohol,the recovery rate of total flavones was 93. 0%,92. 4% and 93. 7% for three methods. And the purity were 15. 84%,12. 71% and 12. 33%,respectively. Extraction ratio of total flavones in burdock leaves raised by cellulase and alcohol extraction method,purification by polyamide column increased the purity dramatically. These data gave a valuable information for further research on burdock leaves.