Food and Fermentation Industries

Isolation and identification of dominant microorganism in traditional fermentative Meidouzha and characterization of dominant strain

  • XU Shu-ze ,
  • HUANG Li ,
  • TENG Jian-wen ,
  • WEI Bao-yao ,
  • XIA Ning
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Online published: 2014-03-25

Abstract

A Meidouzha production strain was selected from traditional fermentative soybean residue. It could produce protease and cellulase. After vaccination,the strain could fermented soybean residue well,and then develop the similar texture and flavor to the Meidouzha. The strain was identified by their morphs of mycerial,hyphal,spore and ITS sequence. The results showed that the strains were Neurospora crassa. Then the physiological property of the strain was studied based on different pH value,temperature,time and weight of the mycelium to define optimum phase of growth of the strain. The range of its log growth phase was 0 ~ 36 h. The range of optimum growth temperature was 28 ~ 32℃. The optimum pH was 6.

Cite this article

XU Shu-ze , HUANG Li , TENG Jian-wen , WEI Bao-yao , XIA Ning . Isolation and identification of dominant microorganism in traditional fermentative Meidouzha and characterization of dominant strain[J]. Food and Fermentation Industries, 2014 , 40(03) : 102 -106 . DOI: 10.13995/j.cnki.11-1802/ts.2014.03.028

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