The antioxidant activity,total phenols and total flavonoids of leatherwood honey were investigated in this study. The results showed that the strong scavenging activity of leatherwood honey for DPPH was concentration dependent. Total phenols content of leatherwood honey was( 473. 74 ± 6. 27) μg/g honey,while total flavonoids content of leatherwood honey was( 0. 375 ± 0. 003) μg/g honey. The total antioxidant activity of leatherwood honey was three times than that of acacia honey,lower than buckwheat honey. Leatherwood honey had a strong protection on DNA damage induced by hydroxyl radicals. This result demonstrated the antioxidant activity of leatherwood honey was related to total phenols.