Thermoactinomyces vulgaris can be applied to starch processing,leather manufacturing and high-temperature compost producing because it can produce various heat resistant enzymes. But identifying T. vulgaris by routine microbiological identification method is time-consuming and inconvenient,so it is essential to establish a rapid and accurate method for the identification of T. vulgaris. In this study one pair of specific primers were designed based on the gyrB gene of Thermoactinomyces vulgaris and a rapid specific PCR assay was established to screen T. vulgaris strains. Experimental results showed that the designed specific primers were specific for Thermoactinomyces vulgaris,and thus were suitable for rapidly screening T. vulgaris strains. The specific PCR method was proved to be more rapid and low-cost compared with traditional identifying methods.