The extraction and purification technology of hydroxytyrosol from olive leaves were studied. Hydroxytyrosol was obtainable by enzymolysis extraction and purified by macroporous adsorption resin. The single factor and orthogonal design were adopted to find out the optimal processing conditions. Results showed that the optimal enzymolysis conditions were determined as follows: pH 7,the enzyme concentration 30 μg / mL and extracting time 80 min at 40℃,the hydroxytyrosol content was 3. 72 mg / g. The experiments of static adsorption and desorption showed that DA-201 macroporous adsorption resin could adsorb and desorb hydroxytyrosol well,and the dynamic optimum technological conditions of the DA-201 were: the solution concentration at 60 μg / mL,the velocity of adsorption 3 BV / h, the eluting reagent 80% ethanol,the eluting velocity 3 BV / h,eluent volume at 2 BV. Under the above optimum experimental conditions,1g olive leaf can obtain 3. 85 mg hydroxytyrosol grease total saponin,the purity of hydroxytyrosol could reach 87. 01%. This method increased purity of hydroxytyrosol and set the foundation for the industrialized production.