As an essential amino acid with branched chain,L-isoleucine( Ile) plays an important role in amino acid market. In order to increase the production and yield of L-isoleucine by Corynebacterium glutamicum YILW,fed batch culture was performed using a two-stage dissolved oxygen control strategy coupled with exponential feeding strategies of the growth limiting nutrients such as core steep liquor and phosphate. The optimal initial concentrations of core steep liquor and phosphate were 35. 0 mL / L and 1. 5 g / L,and the biomass and L-isoleucine production reached 27. 66 g / L and 25. 89 g / L under these conditions. With dual exponential feeding and two-stage dissolved oxygen control strategy,the biomass and L-isoleucine production increased to 32. 29 g / L and 31. 32 g / L after 60 h,which was 16. 73% and 20. 95% higher than those of control,respectively. This work will probably lay a foundation for the control and optimization of L-isoleucine fermentation process on industrial scale.