Glucose is not only widely used in food and medicine industries,but also used as feedstock for biochemical fermentation,such as amino acids,antibiotics and sugar alcohols,etc. There are different ways for glucose production from starch,wherein the method widely accepted by the industry is the one with liquefaction and saccharification using thermostable alpha amylase and glucoamylase. The process is efficient and stable,but could be further improved to reduce the energy consumption and the impurities resulted from high temperature cooking. In this paper, a new process of glucose production directly from granular starch will be introduced.