This paper concerned the influence of several large surface solid matrixes on the antimicrobial lipopeptide production. It aimed to explore the new method to increase antimicrobial lipopeptide yield. Attapulgite,activated carbon and gauze were added to the main medium of Bacillus natto with some designed amount. The inhibition rate to specific indicator were determined to evaluate the yield of antimicrobial lipopeptide and scanning electron microscopy was used to study the characteristics of bacteria adhered on different solid matrixes and the action mode of large surface matrix. The optimal fermentation was found in the attapulgite experimental group. The inhibition rate of the best experimental group was 63. 2%,which was 23. 6% higher than that of control group,followed by activated carbon group with 11. 9% higher than that of the control group and the gauze group with 9. 6% higher than that of the control group. The results from determination of biomass showed that cell concentration of control group was 44. 7% lower than that of the best attapulgite experimental group,38. 5% lower than that of the activated carbon group and gauze group. Bacillus natto formed stable biofilms on attapulgite with a thick-dense tissue and attached with high density.Bacillus natto formed a special fold structure on activated carbon and attached with large number of biofilm-bacteria.Bacillus natto got relatively thin but integrated biofilms on the surface of gauze fiber and also attached with lots of bacteria. Antimicrobial lipopeptide productivity by Bacillus natto was increased obviously by adding large surface solid matrixes into fermentation medium and addition of attapulgite led to the best productivity. The increased yield may due to the addition of the large surface solid matrixes which increased the biomass in the broth and the biofilms formed on the matrixes.