The purpose of this study was to isolate high-yield lipase-producing strains from Sediments of the bottom layer of the Glacier No. 1 in Tianshan Mountains,China. And preliminary identification and investigation on activity of cold-adapted lipase had been carried out. Using the screening medium such as Rhodamine-B,Tween-80,Vitoria blue and glyceryl tributyrate activity assay plate,alkaline cold-adapted strains producing lipase were screened. The growth characteristic of the strains had been studied and the related physiological and biochemical experiments were carried out. Taxonomic identities of isolated strains were determined according to the 16S rRNA gene sequences. 5high-yield lipase- producing strains were obtained. Primary identification showed that 1 strain belonged to the genus Bacillus,4 strains belonged to the genus Pseudomonas. The lipase production capacity of Bacillus was slightly higher than the rest of the strains. 5 high-yield lipase-producing strains were psychrophilic. These 5 strains could tolerate high salinities and be fermented under relative low temperature. 4 strains of them could reach the peak production of enzyme after fermentation for 36 h. The research herein had provided foundation for the development of abundant resources of cold-adapted enzymes.