In order to improve naringinase catalytic performance,covalent immobilization of naringinase using domestic Seplite LX-1000EP resin was studied. Monofactorial experiment and Box-Behnken design were adopted to optimize naringinase immobilization conditions,then the data was analyzed with SAS software version 8. 0. The optimum immobilization conditions were summed up: pH of immobilization system,immobilization temperature,the ration of carrier addition,and immobilized time were 6. 5,26. 6℃,0. 79 g Seplite LX-1000EP resin per 5 mL enzyme solution,and 24 hours,respectively. Under these conditions,the specific activity of immobilized naringinase reached3949. 32 U /g,with an enzyme recovery of 44. 79%,and the protein binding rate was 98. 43%. The results above indicated that Seplite LX-1000EP resin was a good carrier for naringinase immobilization research. This study can provide a basic work for both the industrial production of naringinase and the practical application of this enzyme.