The correlation of organic acid production and antimicrobial activity of Pediococcus acidilactici fermentation was studied. Spot-on-lawn and agar well diffusion method were used to exploit its antimicrobial activity and HPLC was used to analyze organic acids production. The results showed that Pediococcus acidilactici had the highest antimicrobial activity and organic acid production with glucose as carbon source. There was positive correlation between the production of lactic acid,acetic acid,total acid and inhibitory activity( P < 0. 05). The study about organic acid production and antimicrobial activity provided the basis for the development of new biological preservatives.