Lysophosphatidylcholine( LPC) was prepared through alcoholysis of soybean phosphatidylcholine( PC) catalyzed by Lipozyme RM IM in n-hexane. Based on single factor experiments,the optimal ethanolysis conditions were obtained as followed: reaction temperature 40℃,lipase dosage 15%( in mass of PC),solvent ratio 1∶ 2( PC∶ n-hexane,m/v),substrate ratio 1∶3( PC∶ hydroxyl of alcohol,mol/mol),the amount of water 1∶80( PC∶H2O,g /uL). Under these conditions,the conversion of LPC was 93% after 16 h. Based on these optimal conditions,the effect of other alcohols on the ethanolysis of PC was studied. n-butanol and tert-butanol showed no significant changes on ethanolysis,while 1,2-propylene glycol and glycerol showed significant inhibiting on ethanolysis.