The relationship between the number of corn mold and aflatoxin B1 production under different storage conditions was studied in this paper. The corn with initial moisture content of 12. 42%,14. 30%,15. 89%,17. 62%and 19. 88% was stored at 30 ℃ and 75%,84% and 92% relative humidity for 35 days. The total amount of Aspergillusflavus and Penicillium was counted. The aflatoxin B1 was determined by high performance liquid chromatography. The result showed that highest quantities of aspergillusflavus at relative humidity of 75%,84% and 92% were reached when the initial moisture contents of corns were up to 15. 89%,17. 62% and 17. 62%. But for the highest amount of aflatoxin,those were 15. 89%,14. 30%,and 15. 89%,respectively. The growth of aspergillusflavus and its toxin-producing ability were inhibited by other microorganisms in the medium and high humidity condition. When corn was stored at 30 ℃ and 75% relative moisture for 35 days,the total numbers of aspergillusflavus and aspergillus Niger were significantly positive correlation with aflatoxin B1( P < 0. 001,k≥0. 997). The total number of aspergillus Niger on the corn was significantly positive correlation with aflatoxin B1 at 30 ℃ and relative moisture 75%( P <0. 05,k = 0. 921).