Torulopsis glabrata is an important strain for pyruvate production on industrial scale. It mainly utilized glucose as carbon source in previous reports. In the present study,the inexpensive bulk raw material glycerol was used to partially replace the glucose as carbon source. When the medium was supplemented with 80 g /L glucose and20 g /L glycerol,the pyruvate production was 27. 6 g /L in flask culture and 61. 7 g /L in a 7 L fermentor,respectively. Compared to the case without glycerol addition,no obvious change was detected on pyruvate production. Since carbon source was the main component of the medium,it decreased the cost of raw materials during pyruvate accumulation and reduced the problem of excessive production of glycerol by partially replacement of glucose.