In order to improve resistance to various stresses during fermentation of brewing yeast,UV mutagenesis was carried out on industry brewing yeast Y-1. Strain MR0-8 and MR1-2 with obviously improved stress tolerance were isolated on micafungin plate and stressful plates. Compared with the origin strain Y-1,the viability of strain MR0-8 and MR1-2 at the end of fermentation were improved by 19% and 34%,respectively. Further RT-PCR analysis indicated that the expression of genes for stress response and genes in cell wall integrity pathway were higher in MR1-2 than those in Y-1. In response to ethanol and hyperosmolarity stress,most responsive genes were unregulated in Y-1,while the performance of these genes was more complicated in MR1-2.