The effects of chicken leg skin collagen peptide with molecular weight of less than 3 kDa on mushroom tyrosinase and melanin biosynthesis in B16 melanoma cells were studied. The Collagen Peptide inhibitory activity on mushroom tyrosinase and its tyrosinase inhibition kinetics were investigated using L-DOPA as the substrate. Then the safety of chicken collagen peptide extract as well as its effect on the synthesis of melanin was verified using B16 melanoma cells. The results showed that the chicken collagen polypeptide whose molecular weight less than 3 kDa had tyrosinase inhibition activity with an IC50 value of 0. 570 mg /mL. In addition,the inhibition type belonged to noncompetitive inhibition,and the Michaelis constant Km and inhibition constant Kiwere 24. 68 μmol /ml and 10. 76 mg /mL,respectively. Chicken collagen polypeptide in the concentration range of 0 ~ 200 μg /mL had no effect on the proliferation of melanoma cells. Moreover,it could effectively suppress the generation of melanin cells.