To explore adjustable characteristic of permeability of chitosan composite films,the chitosan composite films were prepared by blending method. Nano-SiOxcontent,the ambient temperature and relative humidity( RH) on permeability of the regulation were investigated. It is confirmed that hydrogen bonds emerge between chitosan and SiOxby hybrid materials X-ray diffraction( XRD) and transmission electron microscopy( TEM) analysis. The addition of 0. 03 g /100 mL nano-SiOxwas dispersed well in chitosan composite films,and effect of nano-SiOxcontent to permeability and respiratory intensity was very significant. Adding the same amount of nano-SiOxand lowered the temperature,O2permeability( OP),CO2permeability( CP) and water vapor permeability( WVP) all got decreased lower in certain humidity. The water vapor permeability of chitosan composite films increases with an increase in relative humidity at certain temperature. Nano-SiOxcontent has little effect to light transmittance( LT) of the composite film.It is concluded that OP,CP and WVP of the chitosan composite membranes can be regulated through the control of the amount of nano-SiOx,temperature and relative humidity.