Preparation of alginate-chitosan microcapsules of royal jelly by high-voltage electrostatic field was studied. On the basis of single factor experiments,response surface methodology was applied to optimize the preparation conditions. The loading of obtained royal jelly microcapsules products,10-HDA yield and protein yield were70. 39%,71. 83%,97. 16%,respectively. Under the optimal conditions( the concentration of sodium alginate,chitosan,calcium chloride and royal jelly were 19. 2,5. 8,10. 1 g /L and 150 g /L respectively. The coagulation bath pH was 4. 5. In vitro release study showed the microcapsule of royal jelly was able to block the sour and spicy flavor with enteric solubility.